EN219-alkyne

EN219-alkyne is an alkyne-functionalized EN219 probe. EN219 is a moderately selective synthetic covalent ligand against an N-terminal cysteine (C8) of RNF114 with an IC50 of 470 nM. EN219 inhibits RNF114-mediated autoubiquitination and p21 ubiquitination[1][2]. EN219-alkyne is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

C22H19BrClN3O3

488.76

C#CCNC(COC1=CC=C(C2CC(C3=CC=C(Br)C=C3)=NN2C(CCl)=O)C=C1)=O

Fluorescent Dye

10mM

99.62

DMSO : 100 mg/mL (ultrasonic)

-20°C (Powder, sealed storage, away from moisture)

Blue Ice