Flubida-2

Flubida-2 is a cell permeable dye which can be hydrolyzed to Fubi-2 by endoesterases in cells (after hydrolysis, Ex=492 nm, Em=517 nm) . Flubida-2 can be used to detect pH at a specific site in a cell organelle by directing the probe to where avidin fusion proteins are located[1].

C43H48N4O10S

812.93

O=C(C1=CC=C(C2(OC3=O)C4=CC=C(OC(C)=O)C=C4OC5=CC(OC(C)=O)=CC=C52)C3=C1)N(C)CCCCCCN(C)C(CCCC[C@@]6([H])C(NC(N7)=O)C7CS6)=O.O=C(C8=CC=C(C9=C8)C(OC%109C%11=CC=C(OC(C)=O)C=C%11OC%12=CC(OC(C)=O)=CC=C%12%10)=O)N(C)CCCCCCN(C)C(CCCC[C@@]%13([H])C(NC(N%14)=O)C%14CS%13)=O

Fluorescent Dye

10 mM in DMSO

Room temperature