Fluo-3FF AM [CAS 348079-13-0]

Fluo-3FF AM is a low affinity (Kd = 42 μM) fluorescent Ca2+ sensitive indicator (Abs/Em = 462 nm/526 nm) . Fluo-3FF AM is Mg2+ insensitive and relatively photostable. Fluo-3FF AM is an analog of Fluo-3FF AM. Fluo-3FF AM is essentially non-fluorescent, but exhibits a strong fluorescence enhancement upon entry into cells and binding to calcium.[1]

[348079-13-0]

C50H46Cl2F2N2O23

1151.8

O=C(C)OCOC(CN(CC(OCOC(C)=O)=O)C1=C(OCCOC2=C(C(F)=CC=C2N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)F)C=C(C3=C4C=C(C(C=C4OC5=C3C=C(Cl)C(OCOC(C)=O)=C5)=O)Cl)C=C1)=O

Fluorescent Dye

10 mM in DMSO

Room temperature