Coronaviruses (CoVs) are enveloped, positive-sense, single-stranded RNA viruses with the largest genomes known among RNA viruses. The CoVs S-protein mediates receptor binding and fusion of the viral and host cell membranes. HCoV-229E is an alpha-coronaviruse that uses different host proteins as receptors.In HCoV-229E, its receptor binding domain (RBD) shows extensive sequence variation. HCoV-229E exploits trypsin, cathepsin L and TMPRSS2 to complete the fusion activation mediated by the S protein[1][2]. HCoV-229E Spike/S Protein (APT69883, sf9, His) is the recombinant Virus-derived HCoV-229E Spike/S protein, expressed by Sf9 insect cells , with C-His labeled tag.
