M7 (3'AcmG) (5') ppp (5') (2'OMeA) pU (ammonium)

M7 (3'AcmG) (5') ppp (5') (2'OMeA) pU ammonium is a cap analogue that requires the T7 promoter with AU as the start sequence for mRNA synthesis.

C34H47N13O25P4.xNH3

O=C1C([N+](C)=CN2[C@H]3[C@H](O)[C@H](CNC(C)=O)[C@@H](COP(OP(OP(OC[C@H]4O[C@@H](N(C=N5)C6=C5C(N)=NC=N6)[C@H](OC)[C@@H]4OP(OC[C@H]7O[C@@H](N8C(NC(C=C8)=O)=O)[C@H](O)[C@@H]7O)([O-])=O)(O)=O)(O)=O)(O)=O)O3)=C2N=C(N)N1.N.[x]

DNA/RNA Synthesis

10 mM in H2O

Room temperature