M7 (3'Ma-Cy7) Gppp (2'OMe) ApG ammonium

M7 (3'Ma-Cy7) Gppp (2'OMe) ApG ammonium is a Cy7-labled cap analogue that can be used for mRNA synthesis. The efficiency of mRNA delivery can be determined by the detection of Cy7 by flow cytometry, which can be used to track mRNA in cells and in living animals and to screen delivery lipid ratios.

C68H86N18O30P4S2.xNH3

CC(/C(N1CCCCCC(NC[C@H]2[C@@H](O)[C@H](N(C=[N+]3C)C4=C3C(NC(N)=N4)=O)O[C@@H]2COP(OP(OP(OC[C@H]5O[C@@H](N(C=N6)C7=C6C(N)=NC=N7)[C@H](OC)[C@@H]5OP(OC[C@H]8O[C@@H](N(C=N9)C%10=C9C(NC(N)=N%10)=O)[C@H](O)[C@@H]8O)(O)=O)(O)=O)(O)=O)(O)=O)=O)=C\C=C\C=C\C=C\C%11=[N+](CC)C(C=CC(S(=O)([O-])=O)=C%12)=C%12C%11(C)C)(C)C%13=C1C=CC(S(=O)([O-])=O)=C%13.N.[x]

DNA/RNA Synthesis

10 mM in H2O

Room temperature