Difluorocarboxyfluorescein NHS Ester, 6-isomer [CAS 2702251-82-7]
Difluorocarboxyfluorescein NHS Ester, 6-isomer [CAS 2702251-82-7]
SKU:QM-0010899
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Product Details
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Description
Difluorocarboxyfluorescein NHS Ester, 6-isomer is an amine-reactive fluorinated analog of fluorescein that overcomes some of the key limitations of fluorescein, including greater photostability and a lower pKa (pKa ~ 4.7 versus 6.4 for fluorescein), making its fluorescence essentially pH insensitive in the physiological pH range. Although the mixed isomers of Difluorocarboxyfluorescein NHS Ester, is a preferred, routinely used fluorescent dye for labeling proteins, peptides and nucleotides, purification of peptide and nucleotides labeled with 5 (6) isomers might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer usually give better resolution in HPLC purification that is often required in the conjugation processes.
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CAS Number
[2702251-82-7]
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Molecular Formula
C25H13F2NO9
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Molecular Weight
509.37
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Smiles
O=C(C1=C(C2=C3C=C(C(C=C3OC4=C2C=C(F)C(O)=C4)=O)F)C=C(C(ON5C(CCC5=O)=O)=O)C=C1)O
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Target
Fluorescent Dye
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Shipping Conditions
Room temperature
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Difluorocarboxyfluorescein NHS Ester, 6-isomer [CAS 2702251-82-7]
Difluorocarboxyfluorescein NHS Ester, 6-isomer is a premium-grade research compound supplied by Quantimol for laboratory and scientific applications.