Hoechst 33258 (trihydrochloride) [CAS 23491-45-4]
Hoechst 33258 (trihydrochloride) [CAS 23491-45-4]
SKU:QM-0151739-01
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Product Details
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Description
Hoechst 33258 trihydrochloride is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore, Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution[1].
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CAS Number
[23491-45-4]
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Molecular Formula
C25H27Cl3N6O
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Molecular Weight
533.88
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Smiles
OC1=CC=C(C2=NC3=CC=C(C4=NC5=CC=C(N6CCN(C)CC6)C=C5N4)C=C3N2)C=C1.[H]Cl.[H]Cl.[H]Cl
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Target
Fluorescent Dye
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Concentration
10mM
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Purity
99.71
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Solubility
DMSO : 100 mg/mL (ultrasonic) |H2O : 12.5 mg/mL (ultrasonic; warming; heat to 60°C)
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Storage Conditions
4°C (Powder, sealed storage, away from moisture and light)
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Shipping Conditions
Room Temperature
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Hazard Statement
H302, H315, H319, H335
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Precautions
H302, H315, H319, H335
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Hoechst 33258 (trihydrochloride) [CAS 23491-45-4]
Hoechst 33258 (trihydrochloride) is a premium-grade research compound supplied by Quantimol for laboratory and scientific applications.